Resistance gene cloning from a wild crop relative by sequence capture and association genetics

by Sanu Arora, Burkhard Steuernagel, Kumar Gaurav, Sutha Chandramohan, Yunming Long, Oadi Matny, Ryan Johnson, Jacob Enk, Sambasivam Periyannan, Narinder Singh, M. Asyraf Md Hatta, Naveenkumar Athiyannan, Jitender Cheema, Guotai Yu, Ngonidzashe Kangara, Sreya Ghosh, Les J. Szabo, Jesse A. Poland, Harbans Bariana, Jonathan D.G. Jones, Alison R. Bentley, Mick Ayliffe, Eric Olson, Steven S. Xu, Brian J. Steffenson, Evans Lagudah, Brande B. H. Wulff

Scientific Research Year: 2018 DOI: https://doi.org/10.1038/s41587-018-0007-9

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.